一、 简介

张凡，教授，博士生导师。中国昆虫学会理事会城市昆虫专业委员会委员，山东省动物学会理事，山东健康教育与促进会消毒与媒介生物控制分会副主任委员，山东预防医学会媒介生物学及控制分会委员会常务委员。2007年获浙江大学博士学位，同年至山东师范大学生命科学学院动物学教研室工作至今，2019年-2020年至美国北卡罗来纳州立大学农业与生命科学学院担任访问学者。承担国家自然科学基金6项，省部级项目7项，国家重大科技专项2项，企事业委托横向课题4项。发表相关论文50篇余篇，其中SCI论文40余篇，授权国家发明专利9项，参编教材4部，担任多个SCI杂志Review Editor。主讲普通动物学及实验、行为生态学、保护生物学及生物标本制作等课程，指导学生国家级竞赛获奖5项，省级获奖20余项，具有丰富的奥林匹克生物竞赛辅导经验。

二、 研究方向

1.害虫防治（媒介、粮储、农林害虫等）

2.资源昆虫的开发利用（萤火虫等观赏昆虫及药食昆虫）

3.生物多样性调查和保护（昆虫、鱼类、陆生脊椎动物）

三、 承担项目

纵向课题：

1.山东省自然科学基金：C2H2型锌指蛋白转录因子调控表皮蛋白CPLCP参与德国小蠊高效氯氰菊酯抗性机制（ZR2023MH315）项目起止年月：2024年- 2026年

2.国家自然科学基金：德国小蠊肠道菌分泌的活性肽抗白僵菌感染的作用机制研究（81572027），项目起止年月：2016年-2019年

3.山东省重点研发计划（公益类）：新型系列复配生物灭蟑毒饵的研制和开发（2017GSF221012），项目起止年月：2018年- 2020年

4.山东省自然科学基金：高效氯氰菊酯抗性对德国小蠊生物适合度的影响及其机理研究（ZR2012CQ040）项目起止年月：2012年-2015年

5.山东省高等学校科技发展项目：德国小蠊抗高效氯氰菊酯相关蛋白的分离及鉴定（J11LC13）项目起止年月：2011年-2014年

6. 山东省医药卫生科技发展计划项目：德国小蠊抗药性机理及蛋白分子标记物的筛选研究（2011HZ056）、2011-2013

7. 国家自然科学基金：纤毛在早产儿视网膜早期病变中的病理学功能及分子机制 （31701169）项目起止年月：2018- 2020

8. 国家重大课题专项子课题：人工调控下自然湿地生态恢复技术与工程示范（No.20082x07209-006）、2008-2009

9.国家自然科学基金：非吸烟女性肺癌患者唾液基因标志物图谱与遗传和环境因素相关性的研究（（31471202），项目起止年月：2014-2017

10. 国家自然科学基金：抗辐射菌DNA损伤修复基因的表达及在细胞损伤检测中的应用（30770650），2008-2009

11.国家自然科学基金：家蚕微粒子虫发芽抑制因子的基因克隆与分析（30471311）、2005-2007

横向课题：

1.仰天山国家森林公园生物多样性调查（2024-2025）

职责：陆生脊椎动物多样性全维度调查（两栖类、鸟类、兽类）

2.四川岷江紫坪铺水利工程陆生生态影响评估（2022HXFWSBXY016）

职责：生态修复潜力分析，提出工程优化建议

3.滨州小开河湿地公园生物多样性监测（2016-2018）

职责：水生脊椎动物（两栖类、爬行类、鱼类）动态数据库构建

4.北京市山区生态清洁小流域规划（2014HXFWSBXY013）

职责：2条小流域水生态健康评估（底栖动物、鱼类）

四、 教学科研成果（论文、专著、专利等）

1. Tong Cai, Xuejun Wang , Baorui Liu, Haizheng Zhao, Caixia Liu, Xiancui Zhang, Yuting Zhang, Huiyuan Gao , Coby Schal*, Fan Zhang*.A cuticular protein, BgCPLCP1, contributes to insecticide resistance by thickening the cockroach endocuticle.International Journal of Biological Macromolecules.2024,254,127642 (通讯作者)

2. Haizheng Zhao, Meng Jiang , Xuejun Wang, Huiyuan Gao , Yuting Zhang, Jingjing Wang , Xuexia Zhang*, Dongqin Zhao*, Fan Zhang*.Pathogenic fungi synergistically cooperate with Serratia marcescens to increase cockroach mortality.Pesticide Biochemistry and Physiology.2024,202, 2024, 105951

3. Huiyuan Gao Yanan Zang,Yuting Zhang,Haizheng Zhao,Wenxiao Ma,Xingyu Chen,Jingjing Wang,Dongqin Zhao,xuejun Wang*, Fan Zhang*.Transcriptome analysis revealed that short-term stress in Blattella germanica to β-cypermethrin can reshape the phenotype of resistance adaptation.Pesticide Biochemistry and Physiology.2023,197,105703 (通讯作者)

4. Xiancui Zhang , Huihui Feng, Jintao He, Abrar Muhammad, Fan Zhang*,Xingmeng Lu*.

5. Features and Colonization Strategies of Enterococcus faecalis in the gut of Bombyx mori..Frontiers in Microbiology.2022,13,921330(通讯作者)

6. Xiancui Zhang, Fan Zhang, Xingmeng Lu. Diversity and Functional Roles of the Gut Microbiota in Lepidopteran Insects.2022, Microorganisms, 10(6):1234(通讯作者)

7. Xian Cui Zhang , Meng Jiang, Ya Nan Zang, Hai Zheng Zhao , Cai Xia Liu, Bao Rui Liu , Hua Xue, Coby Schal, Xing Meng Lu, Dong Qin Zhao, Xue Xia Zhang*, Fan Zhang*. Metarhizium anisopliae is a valuable grist for biocontrol in beta-cypermethrin-resistant Blattella germanica (L.).Pest Manag Sci, 2022, doi: 10.1002/ps.6769. (通讯作者)

8. Xiancui Zhang, Huihui Feng,Jintao He,Xili Liang,Nan Zhang,Yongqi Shao, Fan Zhang*,Xingmeng Lu*.The Gut Commensal Bacterium Enterococcus faecalis LX10 Contributes to Defending against Nosema bombycis Infection in Bombyx mori.Pest Manag Sci,2022, https://doi.org/10.1002/ps.6846(通讯作者)

9. Xiaoyan Wu,Shuo Wang, Chen Li, Jianli Shi, Zhe Peng, Chang Liu, Hong Han, Yingru Ma, Limei Zheng, Shaojian Xu, Wei Du1, Jun Li* and Fan Zhang*.CRISPR/Cas9-Mediated Knockout of the Dicer and Ago2 Genes in BHK-21 Cell Promoted Seneca Virus A Replication and Enhanced Autophagy.Front. Cell. Infect. Microbiol., 2022 (通讯作者)

10. Wu XY , Wang S, Xin CX , Li C, Shi JL , Peng Z, Liu C, Han H, Yang Y, Tian Y, Li JX , Xu SJ , Li J*, Zhang F*.Mutation of the sixth amino acid of the Rep protein has no effect on porcine circovirus 2b but enhances porcine circovirus 2d replication in vitro.Archives of Virology. 2021, https://doi.org/10.1007/s00705-021-05217-5 (通讯作者)

11. Jiang M, Dong FY, Pan XY, Zhang YN*,Zhang F*. Boric acid was orally toxic to different instars of Blattella germanica (L.) (Blattodea: Blattellidae) and caused dysbiosis of the, gut microbiota. Pesticide Biochemistry and Physiology. 2021,172:104756 https://doi.org/10.1016/j.pestbp.2021.104756(通讯作者)

12. Yang R A, Zhang MJ,Coby Schal,Cai T,Zhang F*. Boric acid enhances Metarhizium anisopliaevirulence in Blattella germanica (L.) by disrupting the gut and altering its microbial community. Biological Control. 2021, 152:104430(通讯作者)

13. Pan X Y, Wang X J , Zhang F. New Insights into Cockroach Control: Using Functional Diversity of Blattella germanica Symbionts. Insects. 2020 (通讯作者)

14. Chao Y,Wang MY,Dai W,Wang XJ*,Zhang F*.Synergism Between Hydramethylnon and Metarhizium anisopliae and Their Influence on the Gut Microbiome of Blattella germanica (L.).Insects.Inpress, 2020 (通讯作者)

15. Li YR, Coby Schal, Pan XY, Huang YH*, Zhang F *. Effects of Antibiotics on the Dynamic Balance of Bacteria and Fungi in the Gut of the German Cockroach. J Econ Entomol. In press，2020 (通讯作者)

16. Cai T, Huang YH, Zhang F*.Ovarian morphological features and proteome reveal fecundity fitness disadvantages in β-cypermethrin-resistant strains of Blattella germanica (L.) (Blattodea: Blattellidae) . Pestic Biochem Physiol ,In press，2020 (通讯作者)

17. Zhang F, Yang C.L, Zhang X.C, Zhu H.Y, Huang Y.H, et al. Isolation of an anti-entomopathogenic fungal protein secreted from Pseudomonas aeruginosa BGf-2: an intestinal bacteriam of Blattella germanica (L.), Journal of Invertebrate Pathology, 2020（in press）

18. Zhang F, Yang R A. Life history and functional capacity of the microbiome are altered in beta-cypermethrin-resistant cockroaches.International Journal for Parasiology 49(9), 715-723, 2019.

19. Pan X.Y, Zhang F*. Advances in Biological Control of the German cockroach, Blattella germanica (L.).Biological Control, 2020,142:104104 (通讯作者)

20. Zhang XC, Li XX, Gong YW, Li YR, Zhang KL, Huang YH, Zhang F*, Isolation, Identification, and Virulence of a New Metarhizium anisopliae Strain on the German Cockroach. J Econ Entomol. 111(6):2611-2616,2018 doi: 10.1093/jee/toy280, 2019 (通讯作者)

21. Yang C.L, Zhu H.Y, Zhang F*. Comparative Proteomics Analysis Between the Short-Term Stress and Long-Term Adaptation of the Blattella germanica (Blattodea: Blattellidae) in Response to Beta-Cypermethrin. J Econ Entomol .Doi : 10.1093/jee/toz047, 2019 (通讯作者)

22. Zhang X.C, Zhang F*. The Potential Control Strategies Based on the Interaction Between Indoor Cockroaches and Their Symbionts in China. Advances in Insect Physiology, 55, 55-122, 2018 (通讯作者)

23. Zhang F, Sun X X, Zhang X C et al. The interactions between gut microbiota and

24. entomopathogenic fungi: a potential approach for biological control of Blattella germanica (L.). Pest Manag Sci, 74(2):438-447, 2018

25. Zhang F, Wang X.J, Huang Y.H, Zhao Z.G, Zhang S.S, Gong X.S, Xie L, Kang D.M*, Jing X*.Differential Expression of Hemolymph Proteins between Susceptible and Insecticide-Resistant Blattella Germanica. Environmental Entomology, 43(4): 1117-1123, 2014

26. Zhang F, Huang Y.H, Liu S.Z, Zhang L, Li B.T, Zhao X.X, Fu Y, Liu J.J, Zhang X.X*. Pseudomonas reactans, a Bacterial Strain Isolated from the Intestinal Flora of Blattella Germanica with Anti-Beauveria bassiana Activity. Environmental Entomology, 42(3):453-459, 2013

27. Huang Y.H, Wang X.J, Zhang F*, Huo X.B*, Fu R.S, Sun W.B, Kang D.M, Jing X. The Identification of a Bacterial Strain BGI-1 (Intestinal Bacteria of Blattella Germanica) and its Anti-Entomopathogenic Fungi Activity. Journal of Economic Entomology, 106(1):43-49, 2013 (通讯作者)

28. Zhang F, Lu X*, Kumar V.S, Zhu H, Chen H, Chen Z, Hong J. Effects of a novel anti-exospore monoclonal antibody on microsporidial Nosema bombycis germination and reproduction in vitro. Parasitology, 134: 1551-1558, 2007

29. Ma YF, Gong LL, Zhang MQ, Liu XZ, Guo H, Hull JJ, Long GJ, Wang H, Dewer Youssef, Zhang F, He M*, He P*.Two Antenna-Enriched Carboxylesterases Mediate Olfactory Responses and Degradation of Ester Volatiles in the German Cockroach Blattella germanica

30. Agric. Food Chem. Publication Date:March 15, 2023 https://doi.org/10.1021/acs.jafc.2c08488

31. ZhangXL, YanYH, Zhang F, Zhang XR, Xu H, Liu Y, Huang YH. Analysis of the effect of hyaluronic acid on intestinal flora and its metabolites in diabetic mice via high-throughput sequencing and nontargeted metabolomics.Journal of Functional Foods.Volume 103, April 2023, 105496

32. Ming He, Yun-Feng Ma, Huan Guo, Xuan-Zheng Liu, Gui-Jun Long, Qin Wang, Youssef Dewer, Fan Zhang, Peng He. Genome-wide identification and expression pattern analysis of novel chemosensory genes in the German cockroach Blattella germanica.Genomics, 2022 Feb 11;114(2):110310.

33. Li L.L, Xu JW, Yao WC, Yang H.H, Dewer Youssef , Zhang F, Zhu XY, Zhang YN . Chemosensory genes in the head of Spodoptera lituralarvae , Bull Entomol Res, 2021 Feb 26;1-10. doi: 10.1017/S0007485321000109. Online ahead of print.

34. Wang MM, He M, Wang H, Ma YF, Dewer Y, Zhang F, He P.A candidate aldehyde oxidase in the antennae of the diamondback moth, Plutella xylostella (L.), is potentially involved in the degradation of pheromones, plant-derived volatiles and the detoxification of xenobiotics,Pesticide Biochemistry and Physiology. Volume 171, 2021, 104726

35. Xu JW, Zhu XY, Chao QJ, Zhang YJ, Yang YX, Wang RR, Zhang Y, Xie MZ, Ge YT, Wu XL, Zhang F, Zhang YN, Ji L, Xu L. Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae).Insects. 10(6), 2019

36. He P, Ma Y.F, Wang M.M,Wang H, Dewer Y, Abd El-Ghany N. M, Chen G.L,Yang G.Q, Zhang F, He M. Silencing the odorant coreceptor (Orco) disrupts sex pheromonal communication and feeding responses in Blattella germanica:toward an alternative target for controlling insect-transmitted human diseases.Pest Manag Sci. 2020 Nov17. doi: 10.1002/ps.6187. [Epub ahead of print]

37. Zhang YN, Xu JW，Zhang XC, Zhang F , Wu XM et al. Organophosphorus insecticide interacts with the pheromone-binding proteins of Athetis lepigone: Implication for olfactory dysfunction Journal of Hazardous Materials ,Volume 397, 2020, 122777

38. Qiu, Wenbin; Meng, Kai; Liu, Yanyan; Zhang, Yuyu; Wang, Zhao; Chen, Zhi; Yang, Jie; Sun, Wenbo; Guo, Lihui; Ren, Sufang; Chen, Lei; Yang, Guiwen; Zhang, Fan; Shi, Jianli; Li, Jun; Du, Yijun; Yu, Jiang*; Wu, Jiaqiang*.Simultaneous detection of classical PRRSV, highly pathogenic PRRSV and NADC30-like PRRSV by TaqMan probe real-time PCR.Journal of Virological Methods, 2020, 282: 113774. DOI: 10.1016/j.jviromet.2019.113774

39. Zhu XY, Xu JW, Wang DY, Li M, Zhang NN, Zhang F, Zhang YN, Mang DZ et al.Analysis of chemosensory genes in Semiothisa cinerearia reveals sex-specific contributions for type-II sex pheromone chemosensation. Genomics, Volume 112, Issue 6, 2020, Pages 3846-3855

40. Wang S, Xin C, Wu X, Shi J, Peng Z, Sun P, Wang Y, Xu S, Yang Y, Zhang F, Li J. Genetic characterization of Porcine circovirus type 2 from 2013 to 2018 in Shandong Province, China.Vet Med Sci. 2019 Oct 16. doi: 10.1002/vms3.196

41. Li M, Yang Y, Yao Y, Xiang W, Han J, Wang Y, Bai P, Wang J, Zhu G, Man L, Zhang F, Pan L. Isolation and identification of attractants from the pupae of three lepidopteran species for the parasitoid Chouioia cunea Yang. Pest Manag Sci. 2019 Dec 18. doi: 10.1002/ps.5724. [Epub ahead of print]

42. He P, Wang MM, Wang H, Ma YF, Yang S, Li SB, Li XG, Li S, Zhang F, Wang Q, Ran HN, Yang GQ, Dewer Y, He M.Genome-wide identification of chemosensory receptor genes in the small brown planthopper, Laodelphax striatellus. Genomics. 2019 Nov 22. pii: S0888-7543(19)30796-7. doi: 10.1016/j.ygeno.2019.11.016. [Epub ahead of print]

43. He P, Mang D Z, Wan H, Zhang F, et al. Molecular characterization and functional analysis of a Spodoptera exigua ubiquitous carboxylesterase suggested it acted as a cuticle enzyme degradation of ester chemical stimulus[J]. Pesticide Biochemistry and Physiology, 2019

44. TAN Liu-gang, CHEN Zhao-kun, MA Xin-xin, HUANG Qing-hua, SUN Hai-ji, Zhang F, YANG Shao-hua, XU Chuan-tian, CUI Ning. Glycosylation of the hemagglutinin protein of H9N2 subtype avian influenza virus influences its replication and virulence in mice[J]. Journal of Integrative Agriculture.Volume 18, Issue 7, July 2019, Pages 1443-1450

45. Zhao D, Liang L, Wang S, Nakao T, Li Y, Liu L, Guan Y, Fukuyama S, Bu Z, Kawaoka Y, Zhang F, Chen H.Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response.J Virol. Mar 13;91(7). pii: e02215-16. doi: 10.1128/JVI.02215-16, 2017

46. 张凡*. 德国小蠊抗药性机理的研究.生命的化学, 31(1):1-4 , 2011

47. 张凡*. 德国小蠊肠道菌BGI-17的鉴定及抗真菌研究.中国媒介生物学及控制杂志, 23(1):35-37,2012

48. 黄艳红,张凡,付荣恕,刘树真,霍新北*. 德国小蠊肠道菌群分离及抗真菌活性分析. 中国公共卫生, 27(1):129-130,2011

49. 刘浩, 张凡, 黄艳红, 霍新北*.三种抗生素对德国小蠊肠道菌去除效果的研究. 山东师范大学学报(自然科学版), 27(3):115-117,2012

50. 黄艳红, 霍新北, 王钰雅, 张凡*. 五种德国小蠊全虫蛋白提取方法的效果比较. 中国媒介生物学及控制杂志, 23 (3):209-211,2012 (通讯作者)

51. 王学军, 康殿民*, 张凡, 赵志刚. 德国小蠊抗药性与乙酰胆碱酯酶、谷胱甘肽S-转移酶和超氧化物歧化酶活性关系的研究.中国媒介生物学及控制杂志,24 (2):135-137,2013

52. 李六文,张宪翠,夏静，张硕,赵东芹,张凡*.去除共生菌对德国小蠊生物适合度的影响.山东师范大学学报（自然科学版）,31(4):143-147,2016 (通讯作者)

53. 施万里，杨繁，孙晓霞，卢洁，赵东芹，张凡*.多种抗生素对抗药性德国小蠊肠道菌去除效果的研究. 内蒙古大学学报（自然科学版）,2017 (通讯作者)

54. 刘九榕，邵博文，周宇石，刘慧敏，阚硕，薄鹏飞，张凡*.一株沙雷氏菌的分离鉴定及其对德国小蠊的毒力测定.生物学通报.54(3),74-76,2019 (通讯作者)

发明专利：

1. 发明专利1：一种用于蟑螂生物防治的病原真菌，申请号：201611044989.1，发文序号：2016112401973370

2. 发明专利2：一株金龟子绿僵菌转基因菌株及其制备方法与应用， 申请号：201410542584.5，发文序号：2014101500662930

3. 发明专利3：一种以含有金龟子绿僵菌发酵液和氟蚁腙为主要活性成分的杀虫组合物，申请号：201710995446.6 发文序号：2017102302328460 申请日期：2017.10.23

4. 发明专利4.一种复配生物杀虫剂及其制备方法和应用. 申请号：201811118562.0 发文序号：2018122600725540 申请日期：2018.9.26

5. 发明专利5.一种生物源杀虫组合物及应用。申请号:201910917042.4 发文序号：20191223 00861720 申请日期：2019.12.26

6. 发明专利6.一种制备阿胶小分子低肽的方法。专利号:201710144390.3 发文序号：2017031300460900 申请日期：2017.03.10

7. 发明专利7.一种生物源杀虫组合物及其应用。专利号:201910917042.4 发文序号：2019122300861720 申请日期：2019.12.26

8. 发明专利8.一种复配生物杀虫剂及其制备方法。专利号:201910276798.5 发文序号：2019040801555030 申请日期：2019.04.08

9. 发明专利9.一种通过干扰基因提高绿僵菌杀灭德国小蠊的方法及其应用。专利号:202111639617.4 发文序号：2021123002069310 申请日期：2021.12.29

五、 获奖情况

多次获得山东师范大学优秀教学奖以及教学成果奖，“五导”优秀团队指导教师。指导本科生获2015年第九届挑战杯“创青春”˙青岛银行山东省大学生创业大赛金奖，国家银奖，2013年第十三届“挑战杯”山东省大学生课外学术科技作品竞赛省一等奖，2017年第十五届“挑战杯”大学生课外学术科技作品竞赛省特等奖，国家三等奖；2017年山东省第三届“互联网+”大学生创新创业大赛省银奖；至2023年，指导学生获国家大学生创新创业计划立项13项，国家级竞赛获奖5项，省级获奖20余项。